Guide to Reverse Phase SpinColumns Chromatography for Sample Prep
Introduction.
Fast Protein and Peptide Cleanup with C4, C8 or C18 SpinColumns.
When working with protein and peptide samples some purification is usually required, therefore, to remove the salts, solvents, and protecting groups that can interfere with mass spectrometric or spectrophotometric analysis of the peptides, or to trace enrich small quantities of organism water or to isolate products in serum. The C4, C8 & C18 SpinColumns from Harvard Apparatus offer a rapid method for sample cleanup by isolating and/or purifying components of your sample.
The C4, C8 & C18 Spin Columns use reversed-phase chromatography for the separation of polypeptides and proteins. The type of column used is essential to achieving an optimized separation. The reverse phase columns SPE, are ideal for separation of proteins where high resolution of many closely sized components is needed, but it may require increasing non-polar solvents.
Continued in attached guide.
Modes of Separation.
- Step Gradient
- Direct Elution
- Trace Enrichment
Specifications.
C18 Specifications:
| Particle Size | 10 µm |
| Pore Size | 300À |
| Pore Volume | 0.9ml/gm |
| Surface Area | 100 m2/gm |
| %Carbon (w/w) | 8% |
| Silica Class | Type B |
| Acid and Alkali stable | pH 1.5-10 |
C8 Specifications:
| Particle Size | 5 µm |
| Pore Size | 300À |
| Pore Volume | 0.9ml/gm |
| Surface Area | 100 m2/gm |
| %Carbon (w/w) | 5% |
| Silica Class | Type B |
| Acid and Alkali stable | pH 1.5-10 |
C4 Specifications:
| Particle Size | 4.5 µm |
| Pore Size | 300À |
| Pore Volume | 0.9ml/gm |
| Surface Area | 100 m2/gm |
| %Carbon (w/w) | 5% |
| Silica Class | Type B |
| Acid and Alkali stable | pH 1.5-10 |
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