These operating instructions describe the function of the Isolated Heart for Small Rodents (IH-SR), Type 844. They are part of the equipment and should be kept. This apparatus is intended for work on isolated hearts of small rodents like mice, rats, hamsters, or guinea pigs. By exhanging the cannulae and the pump tubing the IH-SR can be adapted to an array of animal hearts.

This apparatus is available in two versions: LH= Langendorff Heart and WH = Working Heart. Both versions allow for easy conversion between constant flow and constant pressure conditions. The WH version of the apparatus allows isolated heart experiments in both LH or WH mode. The preparation of a WH is always done in the retrograde LH mode. Each LH configuration can be upgraded easily to a WH configuration later on.

The isolated perfused heart is a widely used method covering a large range of applications in physiological and pharmacological research as well as in safety pharmacology and toxicology. Although the method is mainly used with acute acting substances it can also be used on hearts of pre-treated animals in conjunction with acute substances.

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